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Proteintech anti nras
Anti Nras, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+nras/KRAS+Antibody/pm41903755-382-66-67
Average 96 stars, based on 188 article reviews
anti nras - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Incubation:

Article Title: Near-Infrared Phototheranostic Iron Pyrite Nanocrystals Simultaneously Induce Dual Cell Death Pathways via Enhanced Fenton Reactions in Triple-Negative Breast Cancer.
Article Snippet: Triple-negative breast cancer (TNBC) is considered more aggressive with a poorer prognosis than other breast cancer subtypes.. Through systemic bioinformatic analyses, we established the ferroptosis potential index (FPI) based on the expression profile of ferroptosis regulatory genes and found that TNBC has a higher FPI than non-TNBC in human BC cell lines and tumor tissues.. To exploit this finding for potential patient stratification, we developed biologically amenable phototheranostic iron pyrite FeS2 nanocrystals (NCs) that efficiently harness near-infrared (NIR) light, as in photovoltaics, for multispectral optoacoustic tomography (MSOT) and photothermal ablation with a high photothermal conversion efficiency (PCE) of 63.1%.

Article Title: Unveiling the role of G-quadruplex structure in promoter region: Regulation of ABCA1 expression in macrophages possibly via NONO protein recruitment.
Article Snippet: The protein was extracted by centrifugation at 4 ◦C at 12,000 xg for 20 min, and the protein concentration was determined and quantified by a BCA protein assay kit (Solarbio, China). .. Samples were separated on a 10 % sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to a polyvinylidene fluoride (PVDF) membrane through wet transfer, and then incubated with the corresponding anti-ABCA1 (Abcam ab18180, 1:1000 dilution), antiNONO (Abcam, ab70335, 1:2000 dilution), anti-BCL-2 (Proteintech, C.-D. Xiao et al. International Journal of Biological Macromolecules 245 (2023) 125443 26593-1-AP, 1:1000 dilution), anti-NRAS (Proteintech, 10724-1-AP, 1:1000 dilution), or anti-GAPDH (Abcam, ab8245, 1:10000 dilution) antibodies for membrane immunoblotting. .. After incubation with the secondary antibodies corresponding to the primary antibodies, an NcmECL ultra-hypersensitive chemiluminescence reagent (NCM Biotech, P10300) was used for imaging and analysis on a Biorad ChemiDoc XRS+ chemiluminescence gel imager (Biorad, USA).

other:

Article Title: Albiflorin attenuates post-myocardial infarction cardiac fibrosis through inhibiting the RAS-ERK/MAPK signaling pathway.
Article Snippet: Background: Myocardial infarction (MI) frequently leads to acute heart failure.. Albiflorin has been shown to exhibit multiple beneficial effects, including anti-inflammatory, anti-apoptotic, and anti-fibrotic activities.. However, its precise molecular mechanisms in MI remain incompletely understood.

Article Title: New mechanistic insights into the RAS-SIN1 interaction at the membrane
Article Snippet: The following antibodies were used: anti-α-tubulin (#ab52866, Abcam), anti-tAKT (#2920, Cell Signaling), anti-pAKT S473 (#4060, Cell Signaling), anti-pERK T202/Y204 (#9106, Cell Signaling), anti-FLAG (#F3165, Sigma Aldrich), anti-GAPDH (#398600, Invitrogen), anti-GST (own antibody), anti-γ-tubulin (#T5326, Sigma Aldrich), anti-His (#MA5-33032, Thermo Fisher), anti-KRAS (#12063-1-AP, Proteintech), anti-NRAS (#EB08365, Erest Biotech) and anti-SIN1 (#2746272, Merck Millipore).

Polyacrylamide Gel Electrophoresis:

Article Title: Unveiling the role of G-quadruplex structure in promoter region: Regulation of ABCA1 expression in macrophages possibly via NONO protein recruitment.
Article Snippet: The protein was extracted by centrifugation at 4 ◦C at 12,000 xg for 20 min, and the protein concentration was determined and quantified by a BCA protein assay kit (Solarbio, China). .. Samples were separated on a 10 % sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to a polyvinylidene fluoride (PVDF) membrane through wet transfer, and then incubated with the corresponding anti-ABCA1 (Abcam ab18180, 1:1000 dilution), antiNONO (Abcam, ab70335, 1:2000 dilution), anti-BCL-2 (Proteintech, C.-D. Xiao et al. International Journal of Biological Macromolecules 245 (2023) 125443 26593-1-AP, 1:1000 dilution), anti-NRAS (Proteintech, 10724-1-AP, 1:1000 dilution), or anti-GAPDH (Abcam, ab8245, 1:10000 dilution) antibodies for membrane immunoblotting. .. After incubation with the secondary antibodies corresponding to the primary antibodies, an NcmECL ultra-hypersensitive chemiluminescence reagent (NCM Biotech, P10300) was used for imaging and analysis on a Biorad ChemiDoc XRS+ chemiluminescence gel imager (Biorad, USA).

Membrane:

Article Title: Unveiling the role of G-quadruplex structure in promoter region: Regulation of ABCA1 expression in macrophages possibly via NONO protein recruitment.
Article Snippet: The protein was extracted by centrifugation at 4 ◦C at 12,000 xg for 20 min, and the protein concentration was determined and quantified by a BCA protein assay kit (Solarbio, China). .. Samples were separated on a 10 % sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to a polyvinylidene fluoride (PVDF) membrane through wet transfer, and then incubated with the corresponding anti-ABCA1 (Abcam ab18180, 1:1000 dilution), antiNONO (Abcam, ab70335, 1:2000 dilution), anti-BCL-2 (Proteintech, C.-D. Xiao et al. International Journal of Biological Macromolecules 245 (2023) 125443 26593-1-AP, 1:1000 dilution), anti-NRAS (Proteintech, 10724-1-AP, 1:1000 dilution), or anti-GAPDH (Abcam, ab8245, 1:10000 dilution) antibodies for membrane immunoblotting. .. After incubation with the secondary antibodies corresponding to the primary antibodies, an NcmECL ultra-hypersensitive chemiluminescence reagent (NCM Biotech, P10300) was used for imaging and analysis on a Biorad ChemiDoc XRS+ chemiluminescence gel imager (Biorad, USA).

Western Blot:

Article Title: Unveiling the role of G-quadruplex structure in promoter region: Regulation of ABCA1 expression in macrophages possibly via NONO protein recruitment.
Article Snippet: The protein was extracted by centrifugation at 4 ◦C at 12,000 xg for 20 min, and the protein concentration was determined and quantified by a BCA protein assay kit (Solarbio, China). .. Samples were separated on a 10 % sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to a polyvinylidene fluoride (PVDF) membrane through wet transfer, and then incubated with the corresponding anti-ABCA1 (Abcam ab18180, 1:1000 dilution), antiNONO (Abcam, ab70335, 1:2000 dilution), anti-BCL-2 (Proteintech, C.-D. Xiao et al. International Journal of Biological Macromolecules 245 (2023) 125443 26593-1-AP, 1:1000 dilution), anti-NRAS (Proteintech, 10724-1-AP, 1:1000 dilution), or anti-GAPDH (Abcam, ab8245, 1:10000 dilution) antibodies for membrane immunoblotting. .. After incubation with the secondary antibodies corresponding to the primary antibodies, an NcmECL ultra-hypersensitive chemiluminescence reagent (NCM Biotech, P10300) was used for imaging and analysis on a Biorad ChemiDoc XRS+ chemiluminescence gel imager (Biorad, USA).



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TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of <t>Nras</t> G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.
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TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of <t>Nras</t> G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.
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TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of <t>Nras</t> G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.
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Image Search Results


TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of Nras G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.

Journal: Aging Cell

Article Title: Loss of p62 Binding Allows TIF ‐ IA Accumulation in Senescence, Which Promotes Phenotypic Changes to Nucleoli and the Senescence Associated Secretory Phenotype

doi: 10.1111/acel.70334

Figure Lengend Snippet: TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of Nras G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.

Article Snippet: Sections were incubated overnight at 4°C with the primary antibodies: rabbit RRN3 (Abcam 251933) and mouse NRas (Santa Cruz Biotechnology sc‐31), diluted 1:500 in antibody diluent (Dako).

Techniques: Animal Model, Immunohistochemistry, Software, MANN-WHITNEY, Comparison